Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Endothelial sprouting in bulk fibrin hydrogels. A) Fluorescent images of embedded MVEC stained red with UEA-1 in gels made with different MVEC:FB ratios and cultured in different medium volumes. B) Quantitation of the average sprout length in bulk gels (n=3). C) Quantitation of the number of sprouts >100 µm in length in bulk gels (n=3). Error bars represent standard deviation of the mean. * indicates statistical significance (*p<0.05, **p<0.01 and ***p<0.001).

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques: Staining, Cell Culture, Quantitation Assay, Standard Deviation

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Influence of cell ratios and culture media volume on total sprouts.

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques:

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Influence of cell ratios and culture media volume on average sprout length.

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques:

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Characterization of modular microtissues. A) Single MVEC:FB microtissues (1:1 and 1:3) under phase contrast showing microtissue morphology and embedded cells immediately after microbead fabrication. B) Single MVEC:FB microtissues (1:1 and 1:3) under fluorescence showing cell viability (green = live cells, red = dead cells). C) Quantitation of cell viability for a population of microtissues. D) Quantitation of total cells per unit volume for a population of microtissues. E) Total DNA in microtissues as a function of time. Best viewed in color. Error bars represent standard deviation of the mean. * indicates statistical significance (*p<0.05).

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques: Fluorescence, Quantitation Assay, Standard Deviation

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Flow cytometry analysis of MVEC and FB cell population in fibrin co-cultures over time. A,B) Mono-culture of MVEC and FB. C- H) MVEC and FB cell population in co-cultures over time. Error bars represent standard deviation of the mean. * indicates statistical significance (*p<0.01).

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques: Flow Cytometry, Standard Deviation

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Sprouting of neovessels from modular microtissues. A) Fluorescence images (green = fibrin, red = MVEC) at day 7 and 14 with different MVEC:FB ratios. B) Quantification of vessel area normalized to microtissue area. C) Box plot of vessel diameter. The solid center line in the box plot represents the median, the dotted center line in the box represents the mean, and the lower and upper boundaries of the box represent the 25th and 75th percentiles, respectively. Whiskers (error bars) above and below the box indicate the 90th and 10th percentiles. Large dots represent outliers.

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques: Fluorescence

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Vessel lumen diameter at day 7 and day 14 in embedded microtissue cultures

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques:

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Inosculation of MVEC neovessels. A) MVEC (red) networks (i) sprouted from microtissues (green) and formed branches (ii) with hollow lumens (iii, iv). B) Serial histological sections showed inosculation of adjacent MVEC vessels.

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques:

Journal: ACS biomaterials science & engineering

Article Title: Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues

doi: 10.1021/acsbiomaterials.6b00274

Figure Lengend Snippet: Microtissues cultured in suspension aggregated to form larger tissue structures. A, B) By day 7, neovessels were evident in tissue masses. C, D) By day 14, networks of vessels had formed in tissue masses made with 1:3 MVEC:FB microtissues, but were less evident in those made at 1:1. E) Fluorescence staining and F) PECAM/CD-31 IHC confirmed that vessels within tissue structures were created by MVEC.

Article Snippet: Human neonatal foreskin microvascular endothelial cells (MVEC) and normal human lung fibroblasts (FB, passage 9–12) were obtained from a commercial vendor (Lonza Inc., Walkersville, MD).

Techniques: Cell Culture, Suspension, Fluorescence, Staining